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A practitioner's view of spectral flow cytometry

Siddhartha Sharma1, Josh Boyer1, Luc Teyton2

  • 1Department of Immunology and Microbiology, Scripps Research, La Jolla, CA, USA.

Nature Methods
|October 3, 2023
PubMed
Summary

No abstract available in PubMed .

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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