[Single cycles of direct and reverse enzymatic reaction illustrated by malate dehydrogenase]

Changes in the duration of self tryptophane fluorescence of soluble cytoplasmic malate dehydrogenase (MDG) and kinetics of chemical transformation in the course of single cycles of direct and reversible reaction L-malate in equilibrium with oxalacetate were recorded. MDG was shown to catalyse the direct and reversible reactions at different conformational states. The reaction coordinates for direct and reversible processes do not coincide in this case.