[Kinetics of the formation of AT-specific DNA-distamin complex]

Kinetics of DNA--distamin complex formation was studied by means of the stop-flow method. It has been found that the binding reaction is much faster than in the case with distamycin A and has only two stages. The differences in kinetic properties of the complexes of distamin and distamycin A with DNA has been interpreted as resulting from the formation of different bonds by protonated positively charged N-containing groups of ligands with negatively charged DNA phosphates.