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Immune responses, therapeutic anti-tumor effects, and tolerability upon therapeutic HPV16/18 E6/E7 DNA vaccination

Shiwen Peng1, Hsin-Fang Tu1, Michelle Cheng1

  • 1Department of Pathology, Johns Hopkins University , Baltimore, Maryland, USA.

Mbio
|October 4, 2023
PubMed
Abstract

Insights

Needle-free delivery of a novel DNA vaccine targeting human papillomavirus (HPV) E6 and E7 proteins enhanced anti-tumor effects. This approach shows promise for treating HPV-related cancers.

Area of Science:

  • Oncology
  • Immunology
  • Vaccinology

Background:

  • Human papillomavirus (HPV) types 16 and 18 are major causes of cervical cancer.
  • The viral oncoproteins E6 and E7 are critical for oncogenic transformation.
  • A DNA vaccine candidate, pBI-11, targeting HPV16/18 E6 and E7, demonstrated therapeutic anti-tumor effects via intramuscular injection.

Purpose of the Study:

  • To evaluate the efficacy of needle-free intradermal delivery of the pBI-11 DNA vaccine using the Tropis biojector.
  • To assess the impact of split-dose, multi-site administration on vaccine delivery and immune response.
  • To determine the translational potential of this enhanced delivery method for HPV-related diseases.

Main Methods:

  • Split-dose, multi-site intradermal administration of the pBI-11 DNA vaccine via the Tropis biojector in a preclinical model.
  • Assessment of vaccine DNA delivery efficiency.
  • Quantification of HPV antigen-specific CD8+ T-cell responses.
  • Evaluation of anti-tumor therapeutic effects.

Main Results:

  • Intradermal delivery via the Tropis biojector increased pBI-11 DNA vaccine delivery compared to needle injection.
  • Split-dose, multi-site administration enhanced HPV antigen-specific CD8+ T-cell responses.
  • Improved anti-tumor therapeutic effects were observed with the Tropis biojector delivery method.

Conclusions:

  • Needle-free, intradermal delivery of the pBI-11 DNA vaccine using the Tropis biojector enhances immune responses and anti-tumor efficacy.
  • This delivery strategy holds translational potential for treating HPV16/18 infections and associated cancers.
  • The Tropis biojector offers a patient-friendly alternative for DNA vaccine administration.
Keywords:
DNA vaccineE6E7HPV-associated cancersHPV16HPV18customized needle-free tropis systemhuman papillomavirusneedle-free biojector

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