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Updated: Jul 30, 2026

Microfabricated Post-Array-Detectors mPADs: an Approach to Isolate Mechanical Forces
Published on: October 1, 2007
Epithelial cell spreading assay on E-cadherin-coated glass or PDMS substrates for microscopy-based analysis of
Lilian Schimmel1, Ivar Noordstra1
1Centre for Cell Biology of Chronic Disease, Institute for Molecular Bioscience, The University of Queensland, St. Lucia, Brisbane, QLD 4072, Australia.
Abstract:
Adherens junctions (AJs) are multi-protein adhesion structures that couple contractile actomyosin networks of epithelial cells within a tissue. Here, we present an epithelial cell spreading assay on E-cadherin-coated glass or polydimethylsiloxane (PDMS) substrates for detailed microscopy-based analysis of cadherin adhesions. We describe steps for preparation of glass coverslips and PDMS gels, E-cadherin coating, and epithelial cell spreading. Epithelial cells can be seeded on E-cadherin-coated surfaces, thereby mimicking AJ formation in X-Y dimension, making it suitable for microscopy analysis. For complete details on the use and execution of this protocol, please refer to Noordstra et al. (2023).1.
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