Related Experiment Video

Updated: Jul 15, 2025

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines
08:50

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

Published on: April 18, 2025

441

Rho GTPase-activating protein 1 promotes hepatocellular carcinoma progression via modulation by

Yanni Xu1,2, Xiaodi Liu3,4, Jincheng Cao1,2

  • 1Department of Ultrasound, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong Province, China.

Hepatology Research : the Official Journal of the Japan Society of Hepatology
|October 4, 2023
PubMed
Abstract

Insights

Rho GTPase-activating protein 1 (ARHGAP1) acts as an oncogene in liver cancer, promoting proliferation and metastasis. Its expression is regulated by circPIP5K1A via sponging miR-101-3p, offering potential therapeutic targets.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • Rho family GTPases regulate critical cell functions, including proliferation and migration.
  • Targeting Rho GTPase regulators like ARHGAP1 is a strategy for hepatocellular carcinoma (HCC) treatment.
  • Understanding ARHGAP1's clinical significance and regulatory mechanisms in HCC is crucial.

Purpose of the Study:

  • To define the clinical significance of ARHGAP1 expression in HCC.
  • To elucidate the regulatory mechanisms of ARHGAP1 in HCC.
  • To investigate the role of the circPIP5K1A/miR-101-3p/ARHGAP1 pathway in HCC progression.

Main Methods:

  • Analysis of ARHGAP1 and miR-101-3p expression in HCC patient data (TCGA).
  • Validation using HCC patient samples and molecular biology techniques (qPCR, Western blotting, dual-luciferase reporter assays).
  • Functional assays (clonality, adhesion, migration, proliferation) to assess pathway involvement.

Main Results:

  • Elevated ARHGAP1 and reduced miR-101-3p correlate with poorer HCC patient survival.
  • MiR-101-3p suppresses HCC cell growth and invasion by targeting ARHGAP1.
  • circPIP5K1A acts as a carcinogenic sponge for miR-101-3p, upregulating ARHGAP1 expression.

Conclusions:

  • ARHGAP1 functions as an oncogene in liver cancer.
  • circPIP5K1A promotes HCC by sponging miR-101-3p, thereby regulating ARHGAP1.
  • The circPIP5K1A/miR-101-3p/ARHGAP1 axis represents a novel regulatory pathway in HCC.
Keywords:
carcinogenic factorhepatologymalignant tumornon-coding RNAsoncogene

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