Related Experiment Video
Updated: Jul 22, 2026

09:51
Generation of Stable Human Cell Lines with Tetracycline-inducible Tet-on shRNA or cDNA Expression
Published on: March 5, 2013
35.4K
Direct screening for effective shRNA with a single mismatch in human cells without laborious cloning
Mayu Tachibana1, Nazumi Ehara1, Shunya Tanikawa1
1Department of Chemistry and Bioengineering, Division of Science and Engineering for Materials, Chemistry and Biology, Graduate School of Engineering, Osaka Metropolitan University, Sugimoto 3-3-138, Sumiyoshi-ku, Osaka 558-8585, Japan.
Bioscience, Biotechnology, and Biochemistry
|October 4, 2023
Abstract:
We have devised a method for the direct screening of efficient short hairpin (sh)RNA molecules in human cells, eliminating the need for the time-consuming process of cloning in Escherichia coli. Our screening suggested that single mismatches to shRNAs can significantly alter their activity.

