Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Mechanisms of Retrovirus-induced Cancers

Mechanisms of Retrovirus-induced Cancers

Retroviruses are RNA viruses that have been shown to cause cancers in diverse species, including chickens, mice, cats, and monkeys. The RNA genomes of these viruses are first reverse-transcribed into single and then double-stranded DNA (dsDNA) copies. This dsDNA called proviral DNA then integrates into the host genome. Subsequently, the host cell transcribes the proviral DNA in concert with the chromosomal DNA. This leads to the production of viral RNA and proteins that assemble at the host...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Decoding the Multi-Component Synergy of Fu Ling Yin Zi for Anti-Oxidative Stress Applications: Formulation Optimization, Molecular Docking, Cell-Based Validation, and 3D-Printed Dysphagia-Friendly Diets.

Foods (Basel, Switzerland)·2026

Same author

Chimeric biohybrid nanovesicles induce immunogenic cell death for targeted and immune-potentiated glioblastoma therapy.

Asian journal of pharmaceutical sciences·2026

Same author

UAV-based phenotyping outperforms visual canopy wilting for evaluating soybean drought tolerance and yield retention under rainfed conditions.

Frontiers in plant science·2026

Same author

Quantitative trait loci analysis identifying novel genomic regions for off-target dicamba tolerance in soybean [Glycine max (L.) Merr.].

BMC plant biology·2026

Same author

Classification-based genomic prediction for early identification of high-yielding and stable soybean genotypes.

Frontiers in plant science·2026

Same author

Angiopep-2-decorated bacterial outer membrane vesicles penetrate the blood-brain barrier for glioblastoma chemo-immunotherapy.

Drug delivery·2026

Search research articles

Related Experiment Video

Updated: Jul 15, 2025

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

Published on: March 8, 2012

A replicative recombinant HPV16 E7 expression virus upregulates CD36 in C33A cells.

Yunting Shao¹, Peng Wang², Yunji Zheng³

¹School of Biomedical Engineering, Dalian University of Technology, Dalian, China.

Frontiers in Microbiology

|October 5, 2023

Summary

Researchers developed a novel method to create replication-competent recombinant viruses expressing high-risk human papillomavirus (HR-HPV) E7 protein. This advancement facilitates studying HPV oncogenesis and developing new HPV vaccines.

Keywords:

Ad4 CD36 HPV16 E7 high-risk HPV recombinant HPV16 E7 expression virus

More Related Videos

Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle

Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle

Published on: February 1, 2017

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions

Published on: July 18, 2013

Related Experiment Videos

Last Updated: Jul 15, 2025

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

Published on: March 8, 2012

Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle

Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle

Published on: February 1, 2017

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions

Published on: July 18, 2013

Area of Science:

Oncology
Virology
Molecular Biology

Background:

High-risk human papillomavirus (HR-HPV) infection is linked to cancer pathogenesis.
The HPV E7 protein is a key target for immunological intervention.
Challenges in HPV cultivation and E7 protein expression hinder research.

Purpose of the Study:

To develop novel methodologies for constructing replication-competent recombinant viral particles expressing HPV E7 protein.
To facilitate further exploration of E7 oncoprotein function and carcinogenic mechanisms.

Main Methods:

Utilized ccdB-Kan screening and Red/ExoCET systems for recombinant viral particle construction.
Infected C33A cells with recombinant virus for continuous HPV16 E7 expression.
Performed transcriptome sequencing (RNA-Seq) to identify differentially expressed genes.

Main Results:

Successfully established replicative recombinant viral particles expressing HPV16 E7.
Observed upregulation of the CD36 gene in infected cells.
Confirmed CD36 gene upregulation is linked to HPV16 E7 expression via qRT-PCR.

Conclusions:

Recombinant virus retains replication and infection abilities, expressing HPV16 E7.
Upregulation of CD36 promotes cell proliferation via PI3K-Akt and p53 pathways.
Provides a foundation for HPV carcinogenesis research and recombinant vaccine development.