Synergistic correlation between host angiogenin and dengue virus replication.

Area of Science:

• Virology
• Molecular Biology
• Cellular Stress Response

Background:

• Dengue virus (DENV) infection is a global health threat.
• DENV pathogenesis heavily relies on host-cellular machinery.
• Mechanistic details of DENV-host interactions remain incompletely characterized.

Purpose of the Study:

• To characterize the mechanistic basis of DENV-induced stress on host cells.
• To investigate the role of the stress modulator ribonuclease Angiogenin in DENV infection.

Main Methods:

• Quantification of Angiogenin levels in DENV-infected cells.
• siRNA-mediated knockdown of Angiogenin in DENV-infected and mock-infected cells.
• Treatment of Huh7 cells with Ivermectin prior to DENV infection.

Main Results:

• Angiogenin levels were upregulated in DENV-infected cells, increasing with viral replication.
• siRNA knockdown of Angiogenin was unsuccessful in DENV-infected cells.
• Ivermectin treatment significantly reduced DENV replication and Angiogenin levels in later stages.

Conclusions:

• Angiogenin upregulation is linked to DENV replication.
• Angiogenin modulation plays a crucial role in DENV replication and pathogenesis.
• Targeting Angiogenin may offer a therapeutic strategy against DENV.