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Related Concept Videos

Tight Junctions01:29

Tight Junctions

5.3K
Tight junctions are molecular seals between cells that prevent the leaking of fluids, ions, and other small solutes across cavities and compartments in multicellular organisms. They are mainly composed of claudin and occludin transmembrane proteins, and other proteins such as tricellulin and JAM (junctional adhesion molecule). All these proteins are 4-pass transmembrane proteins, except JAM, which is a single-pass transmembrane protein belonging to the immunoglobulin superfamily. The...
5.3K
Adherens Junctions01:24

Adherens Junctions

4.8K
Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types –  adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as  epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as  heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic
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Overview of Cell-Cell Junctions01:14

Overview of Cell-Cell Junctions

25.6K
The complex three-dimensional arrangement of cells in any multicellular organism is defined and maintained by interactions of cells with each other and the extracellular matrix. Cell-cell junctions are specialized structures where the multi-protein complexes on one cell interact with the multi-protein complexes on another  cell. These cell junctions are classified  into three main types based on their function — occluding, anchoring, and gap junctions.
Occluding or Tight...
25.6K
Tension Response at Adherens Junctions01:26

Tension Response at Adherens Junctions

2.7K
The adherens junctions that anchor cells together are multi-protein complexes that dynamically adapt to mechanical stimuli such as tensile forces and shear stress. Mechanosensory proteins in these junctions can sense such mechanical stimuli and undergo a shift in their conformation, resulting in an altered function — a process called mechanotransduction.
α-Catenin as a Mechanosensory Protein
The α-catenin of adherens junctions is an allosteric protein with three VH (vinculin...
2.7K
Anchoring Junctions01:03

Anchoring Junctions

3.8K
Anchoring junctions are multiprotein complexes that help cells connect to other cells and the extracellular matrix. Anchoring junctions are present on the lateral and basal surfaces of cells, providing strong and flexible connections. Focal adhesions are often formed due to cell interactions with the ECM substrata, which initiate signal transduction via kinase cascades and other mechanisms. Together, they provide stability and tissue integrity. There are three types of anchoring junctions:...
3.8K
Cell-matrix's Response to Mechanical Forces01:13

Cell-matrix's Response to Mechanical Forces

2.7K
In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
Anchoring junctions mechanically attach a cell to the...
2.7K

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Related Experiment Video

Updated: Jul 14, 2025

Functional Assessment of Intestinal Tight Junction Barrier and Ion Permeability in Native Tissue by Ussing Chamber Technique
06:43

Functional Assessment of Intestinal Tight Junction Barrier and Ion Permeability in Native Tissue by Ussing Chamber Technique

Published on: May 26, 2021

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Claudin-23 reshapes epithelial tight junction architecture to regulate barrier function.

Arturo Raya-Sandino1, Kristen M Lozada-Soto1, Nandhini Rajagopal2

  • 1Department of Pathology, University of Michigan Medical School, Ann Arbor, MI, USA.

Nature Communications
|October 5, 2023
PubMed
Summary
This summary is machine-generated.

Claudin-23 (CLDN23) strengthens the intestinal epithelial barrier by forming complexes with CLDN3 and CLDN4. These protein interactions create unique tight junction pores, regulating permeability and barrier function.

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Related Experiment Videos

Last Updated: Jul 14, 2025

Functional Assessment of Intestinal Tight Junction Barrier and Ion Permeability in Native Tissue by Ussing Chamber Technique
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Sensing of Barrier Tissue Disruption with an Organic Electrochemical Transistor
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Sensing of Barrier Tissue Disruption with an Organic Electrochemical Transistor

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Area of Science:

  • Cell Biology
  • Biophysics
  • Gastroenterology

Background:

  • Tight junction proteins, claudins, regulate epithelial barrier function.
  • Heterogeneity in gastrointestinal barrier function is linked to differential claudin expression.

Purpose of the Study:

  • Investigate the role of claudin-23 (CLDN23) in intestinal epithelial barrier function.
  • Elucidate the mechanisms by which CLDN23 modulates paracellular permeability.

Main Methods:

  • Utilized complementary experimental approaches to study CLDN23 function.
  • Employed computational modeling to analyze claudin complex formation and pore architecture.

Main Results:

  • CLDN23 is enriched in luminal intestinal epithelial cells, enhancing barrier integrity.
  • CLDN23 associates with CLDN3 and CLDN4, influencing their localization and regulating ion/macromolecule permeability.
  • Computational models revealed that CLDN23-CLDN3/CLDN4 complexes form unique pores with distinct charge and architecture.

Conclusions:

  • CLDN23 strengthens the epithelial barrier by interacting with CLDN3 and CLDN4.
  • Claudin complex formation is interaction-dependent, leading to diverse pore properties.
  • A model is proposed where distinct claudin complexes modulate epithelial barrier function through altered tight junction structure.