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Efficient ilamycins production utilizing Enteromorpha prolifera by metabolically engineered Streptomyces atratus
Yu-Xi Jiang1, Gao-Fan Zheng1, Long-Chao Chen1
1State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China.
Biotechnology for Biofuels and Bioproducts
|October 5, 2023
Summary
Enteromorpha prolifera powder, a sustainable biomass, enhances ilamycin production in Streptomyces atratus. This eco-friendly nitrogen source significantly boosts the yield of these anti-TB cyclic heptapeptides.
Area of Science:
- Biotechnology
- Marine Microbiology
- Chemical Engineering
Background:
- Green tides generate large amounts of Enteromorpha biomass requiring sustainable utilization.
- Ilamycins are novel cyclic heptapeptides with potent anti-tuberculosis (anti-TB) activity, isolated from deep-sea Streptomyces atratus.
- Optimizing fermentation processes is crucial for cost-effective production of valuable biomolecules.
Purpose of the Study:
- To evaluate Enteromorpha prolifera (EP) powder as an eco-friendly nitrogen source for ilamycin production.
- To optimize the fermentation conditions using EP powder for enhanced ilamycin yield.
- To investigate the potential of genetically engineered strains for improved ilamycin biosynthesis.
Main Methods:
- Enteromorpha prolifera powder was utilized as a nitrogen source in the fermentation medium.
- Strain engineering of Streptomyces atratus SCSIO ZH16 was performed, including the construction of mutant strain S. atratus SCSIO ZH16 ΔR::bldD.
- Ilamycin production titers were measured using high-performance liquid chromatography (HPLC) in shake flasks and a 5 L bioreactor.
Main Results:
- Using EP powder as a nitrogen source, ilamycin production reached 709.97 mg/L with the engineered strain S. atratus SCSIO ZH16 ΔR.
- The mutant strain S. atratus SCSIO ZH16 ΔR::bldD with EP powder achieved a significantly higher ilamycin production titer.
- In a 5 L bioreactor, the production titer of ilamycins and ilamycin E reached 1561.77 mg/L and 745.44 mg/L, respectively.
Conclusions:
- Enteromorpha prolifera powder is a viable and sustainable nitrogen source for industrial ilamycin production.
- Strain engineering combined with EP powder optimization offers a promising strategy for enhancing the yield of anti-TB compounds.
- This approach contributes to the circular economy by valorizing marine biomass for pharmaceutical production.
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