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Laser Machined Fiber-based Microprobe: Application in Microscale Electroporation.

Jongwoon Kim1, Yajun Zhao2, Shuo Yang1

  • 1Bradley Department of Electrical and Computer Engineering, Virginia Tech, Blacksburg, VA 24060, USA.

Advanced Fiber Materials
|October 6, 2023
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel fiber-based microprobe for in vivo microscale electroporation. This flexible device enables targeted cell electroporation in sensitive areas like the deep brain.

Keywords:
fiber probeirreversible electroporationmicromachinemicroscale electroporationpolymerreversible electroporation

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Area of Science:

  • Biomedical Engineering
  • Neuroscience
  • Cell Biology

Background:

  • Microscale electroporation is typically limited to in vitro settings using microchannels or microcapillaries.
  • In vivo applications require minimally invasive devices capable of precise cellular targeting.

Purpose of the Study:

  • To develop and validate a flexible, fiber-based microprobe for in vivo microscale electroporation.
  • To enable targeted electroporation in space-sensitive anatomical regions.

Main Methods:

  • Fabrication using thermal drawing and femtosecond laser micromachining.
  • Integration of four copper electrodes (80 μm) and a microfluidic channel (30 μm) within a 400 μm diameter fiber.
  • Validation through numerical simulations and in vitro experiments on U251 human glioma cells in a 3D collagen scaffold.

Main Results:

  • Demonstrated successful reversible and irreversible microscale electroporation.
  • Customizable electrode and channel dimensions achieved via femtosecond laser.
  • Ablation regions accurately estimated and compared with simulations.

Conclusions:

  • The fiber-based microprobe is feasible for in vivo microscale electroporation.
  • The device offers precise cellular targeting in confined environments like the deep brain.
  • This technology advances possibilities for in vivo cellular manipulation and research.