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Scattering And Absorption of Light in Planetary Regoliths
Published on: July 1, 2019
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Mapping optical scattering properties to physical particle information in singly and multiply scattering samples.
Taylor M Cannon1,2, Brett E Bouma1,2, Néstor Uribe-Patarroyo2
1Massachusetts Institute of Technology, Institute of Medical Engineering and Science, 70 Massachusetts Avenue, Cambridge, MA 02141, USA.
Biomedical Optics Express
|October 6, 2023
Summary
Optical coherence tomography (OCT) can reveal tissue microstructure by analyzing light scattering. This study calibrates OCT systems to accurately measure scattering properties, improving diagnostic capabilities for tissues and tumors.
Area of Science:
- Biomedical Optics
- Optical Coherence Tomography
- Tissue Microstructure Analysis
Background:
- Optical coherence tomography (OCT) uses light scattering for endogenous contrast in biological tissues.
- Tissue optical properties, reflecting microstructural details, dictate light scattering behavior.
- Measuring these properties from OCT intensity data can enhance diagnostic applications.
Purpose of the Study:
- To develop and validate a calibrated OCT system for accurate measurement of tissue optical properties.
- To decouple the effects of particle size and concentration on OCT signal attenuation.
- To differentiate between single and multiple scattering in biological samples.
Main Methods:
- Experimental calibration of OCT system geometry (NA, focal plane).
- Measurement of depth-resolved attenuation coefficients and layer-resolved backscattering coefficients.
- Analysis of tissue-mimicking phantoms and murine brain tissue samples.
Main Results:
- OCT system geometry minimally impacts attenuation coefficients in singly scattering samples but significantly affects highly scattering samples.
- Accurate retrieval of scattering particle diameter and concentration was demonstrated in phantoms.
- Tumor-bearing regions in murine brain tissue were identified by increased scattering particle density.
Conclusions:
- Calibrated OCT systems provide enhanced analysis of tissue microstructure by accurately measuring scattering properties.
- Decoupling particle size and concentration improves diagnostic information from OCT.
- Discriminating multiple scattering through system topology adjustments offers a framework for assessing measurement accuracy.
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