Lipopolysaccharide penetration analysis of two different resin cement systems to dentin and ceramic surface

One of the challenges in adhesive ceramic restorations is deterioration of tooth-cement-ceramic interfaces. This study was to quantitatively investigate lipopolysaccharide (LPS) penetration through adhesive ceramic restorations.

Standardized holes were formed on ceramic (IPS Empress CAD, Ivoclar Vivadent) and human dentin discs. Prepared discs were randomly assigned to experimental groups (n = 6/group): SC, self-adhesive resin cement (SmartCem 2, Dentsply Sirona) bonded to ceramic discs; ST, self-adhesive resin cement bonded to dentin discs; VC, etch-and-rinse resin cement (Variolink II, Ivoclar Vivadent AG) bonded to ceramic discs; VT, etch-and-rinse resin cement bonded to dentin discs. The specimens underwent thermocycling (10,000 cycles; 5-55 °C) then LPS penetration test until 5-weeks. A mixed effect analysis using R statistical language was performed for data analysis.

Dentin and ceramic bonded with etch-and-rinse resin cements (groups VT and VC) showed significantly less penetration than those with self-adhesive resin cement (groups ST and SC) (P < 0.05). ST showed significantly lower penetration than SC (P < 0.05), that showed similar penetration with positive control (P > 0.05). VC showed minimal penetration during observed time of period (P < 0.05). Scanning electron microscope observations showed different interfacial characteristics among the groups.

Within the limitation of this study, etch-and-rinse resin cement showed less LPS penetration than self-adhesive resin cement on both ceramic and dentin surfaces.