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Related Experiment Videos

A stable liquid control material for urinary enzyme assays.

D Maruhn, K Wehling, U Metz

    Clinica Chimica Acta; International Journal of Clinical Chemistry
    |October 31, 1986
    PubMed
    Summary

    A stable liquid control material for urinary enzyme assays was developed using ethylene glycol or glycerol. This control material demonstrated stable enzyme activities for 375 days at 4 and -20°C, proving effective for quality control.

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    Lancet (London, England)·1997

    Area of Science:

    • Clinical Chemistry
    • Biochemistry
    • Urology

    Background:

    • Urinary enzyme assays are crucial for diagnosing various conditions.
    • A need exists for stable, reliable liquid control materials for these assays.
    • Current control materials may lack long-term stability or require complex preparation.

    Purpose of the Study:

    • To develop a stable liquid control material for urinary enzyme assays.
    • To assess the long-term stability of key urinary enzyme activities in the developed material.
    • To validate the material's utility in external quality control programs.

    Main Methods:

    • Centrifuged human urine was stabilized with ethylene glycol or glycerol (30% v/v).
    • The pH was adjusted to 7.0 to create the liquid control material.

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  • Enzyme activities (beta-N-acetylglucosaminidase, alanine aminopeptidase, gamma-glutamyltransferase, lactate dehydrogenase) were measured over 375 days at 4°C and -20°C.

    • Main Results:

    • The prepared liquid control material exhibited stable activities for beta-N-acetylglucosaminidase, alanine aminopeptidase, gamma-glutamyltransferase, and lactate dehydrogenase.
    • Stability was maintained for 375 days at both refrigerated (4°C) and frozen (-20°C) conditions.
    • The material successfully passed evaluation in an external quality control program.

    Conclusions:

    • A stable, long-lasting liquid control material for urinary enzyme assays can be prepared using ethylene glycol or glycerol.
    • This material provides a reliable tool for monitoring the accuracy of urinary enzyme measurements.
    • The developed control material is suitable for routine use in clinical laboratories and external quality assessment.