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Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems

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Metasurface-assisted Lab-on-fiber optrode for highly sensitive detection of vitamin D.

A M Cusano¹, G Quero², P Vaiano³

¹Centro Regionale Information Communication Technology (CeRICT) scrl, I-82100, Benevento, Italy.

Biosensors & Bioelectronics

|October 6, 2023

Summary

A new lab-on-fiber biosensor platform offers sensitive, label-free detection of 25-hydroxyvitamin D3. This innovation paves the way for rapid, user-friendly point-of-care diagnostic devices for vitamin D status assessment.

Keywords:

Biosensing Lab-on-Fiber technology Optical fiber biosensors Optical fiber metatips Plasmonic metasurfaces Vitamin D detection

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Area of Science:

Biomedical Engineering
Analytical Chemistry
Nanotechnology

Background:

Vitamin D status assessment requires sensitive, rapid, and user-friendly methods.
Detecting 25-hydroxyvitamin D3 (25(OH)D3) is challenging due to complex sample preparation and its small size.
Point-of-care (PoC) devices need efficient detection strategies for vitamin D metabolites.

Purpose of the Study:

To develop a novel label-free Lab-on-Fiber biosensing platform for sensitive 25(OH)D3 detection.
To optimize bio-functionalization for specific small molecule detection.
To create a foundation for new, low-cost PoC diagnostic devices.

Main Methods:

Integration of plasmonic metasurfaces (MSs) on an optical fiber (OF) tip.
Development of a dedicated pipeline for sensor tip bio-functionalization.
Label-free detection of 25(OH)D3 in buffer and complex matrices.

Main Results:

Achieved highly sensitive detection of 25(OH)D3 in clinically relevant ranges (4-160 ng/mL).
Demonstrated limits of detection (LOD) of 1.40 ng/mL in saline buffer and 0.85 ng/mL in complex matrix.
Confirmed specific detection of small molecules in a label-free configuration.

Conclusions:

The MS-assisted Lab-on-fiber platform offers sensitive and specific detection of 25(OH)D3.
The platform's miniaturization and sensitivity are suitable for PoC diagnostic applications.
This technology presents a promising alternative to conventional methods for vitamin D testing.