Related Experiment Video

Updated: Jul 14, 2025

Chemical Inactivation of the E3 Ubiquitin Ligase Cereblon by Pomalidomide-based Homo-PROTACs
10:44

Chemical Inactivation of the E3 Ubiquitin Ligase Cereblon by Pomalidomide-based Homo-PROTACs

Published on: May 15, 2019

13.3K

βTrCP1 promotes SLC35F2 protein ubiquitination and inhibits cancer progression in HeLa cells

Jencia Carminha Colaco1, Arun Pandian Chandrasekaran1, Janardhan Keshav Karapurkar1

  • 1Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul, 04763, South Korea.

Biochemical and Biophysical Research Communications
|October 6, 2023
PubMed

Insights

Beta-transducin repeat-containing protein 1 (βTrCP1) targets solute carrier family 35 F2 (SLC35F2) for degradation, acting as a tumor suppressor. Depleting βTrCP1 enhances SLC35F2 levels and promotes cancer progression.

Area of Science:

  • Molecular biology
  • Cancer research
  • Biochemistry

Background:

  • Solute carrier family 35 F2 (SLC35F2) is implicated in cancer progression.
  • Understanding SLC35F2 regulation is crucial for cancer therapy.

Purpose of the Study:

  • To identify the E3 ligase responsible for SLC35F2 protein degradation.
  • To investigate the role of βTrCP1 in regulating SLC35F2 levels and cancer cell behavior.

Main Methods:

  • Co-immunoprecipitation to identify interacting proteins.
  • Western blotting to assess protein levels and half-life.
  • Cellular assays to evaluate cell growth, migration, invasion, and colony formation.

Main Results:

  • Beta-transducin repeat-containing protein 1 (βTrCP1) directly interacts with and ubiquitinates SLC35F2, reducing its protein half-life.
  • A negative correlation was observed between βTrCP1 and SLC35F2 mRNA expression in cancer cell lines.
  • βTrCP1 depletion led to SLC35F2 accumulation and enhanced cancer cell phenotypes (growth, migration, invasion, colony formation).

Conclusions:

  • βTrCP1 functions as a tumor suppressor by degrading SLC35F2.
  • βTrCP1 depletion promotes SLC35F2-mediated carcinogenesis.
  • βTrCP1 represents a potential therapeutic target for cancers involving SLC35F2.
Keywords:
Post-translational modificationsProtein destabilizationProtein ubiquitinationProteolysisWound healing

More Related Videos

In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells09:40

In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells

Published on: November 2, 2017

7.3K
Through the Looking Glass: Time-lapse Microscopy and Longitudinal Tracking of Single Cells to Study Anti-cancer Therapeutics06:00

Through the Looking Glass: Time-lapse Microscopy and Longitudinal Tracking of Single Cells to Study Anti-cancer Therapeutics

Published on: May 14, 2016

11.1K

Related Concept Videos

The Retinoblastoma Gene01:20

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.1K
Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
11.6K
Small GTPases - Ras and Rho01:24

Small GTPases - Ras and Rho

Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
4.0K
ER Retrieval Pathway01:45

ER Retrieval Pathway

In the secretory pathway, vesicles transport proteins from one cellular compartment to another in forward transport to deliver the protein to its correct location. Occasionally, misfolded proteins and incorrect proteins escape their original compartments, and a retrieval pathway is used to return the escaped proteins to their original compartment.
The ER uses many checkpoints to prevent the entry of incorrectly folded or a resident protein as cargo onto a transport vesicle. These mechanisms...
3.9K
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
2.1K
Targeted Cancer Therapies02:57

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
7.7K