Gentle ions for cryo-FIB milling
Xiaofeng Chu1, Misha Kudryashev2
1Max Delbrück Center for Molecular Medicine in the Helmholtz Society, Berlin, Germany.
Structure (London, England : 1993)
|October 6, 2023
Abstract:
Cryo-EM imaging of vitreous samples is limited to a few hundred nanometers in thickness. Focused ion beams can mill windows into cells and tissues for imaging, but they damage biological samples. In this issue of Structure, Yang et al. (2023) quantitatively describe this damage and suggest ways to minimize it.
