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Updated: Jul 14, 2025
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Murine Model of Hindlimb Ischemia
Published on: January 21, 2009
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Animal experimental models of ischemic limbs - A systematic review
Veronika Lovasova1, Robert Bem2, Jaroslav Chlupac3
1Transplant Surgery Department, Institute for Clinical and Experimental Medicine, Prague, Czech Republic; Second Faculty of Medicine, Charles University, Prague, Czech Republic.
Vascular Pharmacology
|October 6, 2023
Summary
This review summarizes animal models for ischemic limbs, finding mice most common. Gradual occlusion methods, like two-stage ligation or ameroid constrictors, better mimic human chronic limb ischemia.
Area of Science:
- Biomedical research
- Preclinical studies
- Animal models
Background:
- Limb ischemia research requires reliable animal models.
- Understanding the advantages and disadvantages of existing models is crucial for effective study design.
- This review systematically evaluates animal models for ischemic limbs.
Approach:
- A comprehensive literature review was performed using PubMed and Web of Science.
- Various experimental animals and surgical techniques for inducing limb ischemia were analyzed.
- Key characteristics, including animal species, surgical methods, and ischemia duration, were evaluated.
Key Points:
- Mice, rabbits, and rats are frequently used experimental animals.
- Femoral artery ligation/excision is a common method, but often creates acute ischemia.
- Methods like two-stage ligation, ameroid constrictors, and endovascular techniques offer more gradual occlusion.
Conclusions:
- Mice are prevalent in limb ischemia research.
- Gradual occlusion models (two-stage, ameroid constrictors, endovascular grafts) better represent human chronic ischemia.
- Appropriate animal strain selection and extended observation periods (≥14 days, preferably 4 weeks) are vital for chronic ischemic limb research.
