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Related Concept Videos

Parameters Affecting Nonlinear Elimination: Zero-Order Input, First-Order Absorption and Two-Compartment Model01:13

Parameters Affecting Nonlinear Elimination: Zero-Order Input, First-Order Absorption and Two-Compartment Model

Drugs administered through various routes can lead to nonlinear elimination, resulting in complex pharmacokinetic behaviors crucial to understanding efficacious drug dosing.
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Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification
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Engineered Test Tissues: A Model for Quantifying the Effects of Cryopreservation Parameters.

Jonian Grosha1, Jun-Hung Cho2, Shannon Pasley2

  • 1Worcester Polytechnic Institute, Worcester, Massachusetts 01609, United States.

ACS Biomaterials Science & Engineering
|October 6, 2023
PubMed
Summary

Preserving engineered tissues is crucial for their use in regenerative medicine. This study introduces a new method to screen cryopreservation agents and conditions, improving tissue storage and recovery.

Keywords:
cryopreservationcryoprotective agentsengineered tissuestissue storage

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Area of Science:

  • Tissue engineering
  • Cryobiology
  • Regenerative medicine

Background:

  • Engineered tissues hold promise for in vivo repair and in vitro therapeutic discovery.
  • A significant challenge in tissue engineering is preserving tissue viability, structure, and function during storage and transport.
  • Current cryopreservation agents (CPAs) are primarily designed for cells, with limited options specifically for tissues.

Purpose of the Study:

  • To develop and validate a high-throughput screening method for evaluating commercially available CPAs and cryopreservation parameters for engineered tissues.
  • To establish a standardized approach for assessing tissue viability, morphology, and mechanical properties post-cryopreservation.
  • To facilitate the optimization of cryopreservation protocols for improved engineered tissue storage and recovery.

Main Methods:

  • Utilized engineered tissue rings as standardized test tissues with consistent morphology and mechanical properties.
  • Developed a method for cryopreserving tissues in standard cryovials after 7 days of in vitro culture.
  • Quantified post-thaw outcomes including metabolic recovery, apoptosis/necrosis, morphology, and mechanical integrity.

Main Results:

  • Successfully established a flexible, high-throughput method for screening CPAs and cryopreservation parameters.
  • Demonstrated the ability to assess multiple critical parameters of tissue health and function post-cryopreservation.
  • Identified different CPA formulations, freezing rates, and freezing points that impact tissue recovery.

Conclusions:

  • The developed method enables efficient preliminary screening of cryopreservation strategies for engineered tissues.
  • This approach can accelerate the optimization of protocols to enhance the long-term storage and viability of engineered tissues.
  • The findings contribute to overcoming a major hurdle in the clinical translation and application of engineered tissues.