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Implementation of an Alternative Training Method for Cardiac Blood Collection in Mice.

April J George1, Carly I O'Malley2, Rachel Eb Bulock3

  • 1Learning and Development, Charles River Laboratories, Mattawan, Michigan.

Journal of the American Association for Laboratory Animal Science : JAALAS
|October 6, 2023
PubMed
Summary

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A new, low-cost training model significantly reduces the number of mice needed for cardiac blood collection competency. This animal-free approach improves skill acquisition and trainee confidence, aligning with the 3Rs principles.

Area of Science:

  • Veterinary Medicine
  • Animal Welfare Science
  • Laboratory Animal Science

Background:

  • Training personnel for animal procedures presents logistical and welfare challenges.
  • Cardiac blood collection in mice often requires repeated practice, impacting animal welfare and the 3Rs principles (refinement, replacement, reduction).
  • A non-animal training model is crucial for promoting the 3Rs in animal research training.

Purpose of the Study:

  • To develop and evaluate a cost-effective, non-animal training model for cardiac blood collection in mice.
  • To reduce the number of live animals used in training and improve trainee competency.
  • To enhance the culture of care and refine training methodologies.

Main Methods:

  • A novel training model was constructed using readily available materials at a low cost (<$15 USD per model).

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  • Two concurrent training curricula were compared: an alternative group using the model before live mice, and a traditional group using only euthanized mice.
  • Key variables measured included mice usage, competency rates, time to competency, skill quality, comprehension, feedback, and costs.

    • Main Results:

    • The alternative training group used at least 10 fewer mice per technician compared to the traditional group.
    • Competency rates were higher in the alternative group (82%) versus the traditional group (60%).
    • Skill comprehension and performance quality were superior in the alternative group, indicated by fewer necropsy lesions.

    Conclusions:

    • The developed non-animal model effectively enhances skill development and reduces live mouse utilization in training.
    • This model improves competency, confidence, and addresses compassion fatigue among trainees.
    • Implementing this model as a standard training approach promotes the 3Rs and animal welfare.