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Related Experiment Video

Updated: Jul 14, 2025

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

Published on: May 18, 2017

Deconvolution reveals cell-type-specific transcriptomic changes in the aging mouse brain.

Yingxue Ren¹, Xue Wang², Shuwen Zhang³

¹Department of Quantitative Health Sciences, Mayo Clinic, 4500 San Pablo Road South, Jacksonville, FL, 32224, USA. ren.yingxue@mayo.edu.

Scientific Reports

|October 6, 2023

Summary

This summary is machine-generated.

Aging significantly impacts Alzheimer's disease (AD) pathogenesis. This study reveals how specific brain cell types, including astrocytes and microglia, contribute to aging-driven molecular pathways in AD.

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Last Updated: Jul 14, 2025

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

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Published on: May 18, 2017

Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing

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Published on: December 3, 2019

Microdissection of Mouse Brain into Functionally and Anatomically Different Regions

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Published on: February 15, 2021

Area of Science:

Neuroscience
Genomics
Aging Research

Background:

Aging is a critical factor in Alzheimer's disease (AD) development.
Previous studies identified aging-driven molecular pathways but lacked cell-type specificity.
Understanding cell-type contributions is vital for elucidating AD pathogenesis.

Purpose of the Study:

To dissect cell-type-specific gene expression in aging brain.
To identify the cellular origins of aging-associated molecular pathways in AD.
To analyze interactions between cell types in aging brain transcriptomics.

Main Methods:

Computational deconvolution of bulk brain transcriptomics data.
Analysis of gene expression profiles in astrocytes, microglia, oligodendroglia, neurons, and vascular cells.
Validation using published single-cell RNA sequencing studies.

Main Results:

Immune genes predominantly in microglia, oligodendroglia, and vascular cells.
Lipid metabolism genes mainly in astrocytes, microglia, and oligodendroglia.
Mitochondrial genes prominent in vascular cells; synapse genes in neurons and vascular cells.
Identified intra- and inter-cell-type interactions and validated aging-associated changes.

Conclusions:

Aging-driven molecular pathways in AD involve specific contributions from distinct brain cell types.
Cellular-level analysis provides deeper insights into AD pathogenesis.
Findings highlight the importance of cell-type-specific investigations in neurodegenerative disease research.