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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
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Sanger Sequencing01:57

Sanger Sequencing

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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Maxam-Gilbert Sequencing01:05

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In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
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Genomics02:02

Genomics

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Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
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Updated: Jul 14, 2025

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq
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Next-Generation Sequencing to Study the DNA Interaction.

Nachammai Kathiresan1, Srinithi Ramachandran2, Langeswaran Kulanthaivel1,3

  • 1Department of Biotechnology, Alagappa University, Karaikudi, Tamil Nadu, India.

Methods in Molecular Biology (Clifton, N.J.)
|October 6, 2023
PubMed
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Next-generation sequencing (NGS) revolutionizes genomics, enabling rapid DNA and RNA analysis. This technology drives discoveries in disease, personalized medicine, and environmental science.

Keywords:
CancerDNA interactionInfectious diseaseNext-generation sequencingSequencing

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Area of Science:

  • Genomics
  • Molecular Biology
  • Biotechnology

Background:

  • Next-generation sequencing (NGS) offers high-speed, accurate, and cost-effective DNA and RNA sequencing.
  • Significant advancements in NGS platforms like Illumina, PacBio, and Oxford Nanopore have occurred over the past decade.
  • NGS technologies are crucial for investigating DNA interactions and biological processes.

Purpose of the Study:

  • To highlight the transformative impact of NGS on genomics research.
  • To underscore the role of NGS in identifying genetic variations and disease-associated modifications.
  • To emphasize NGS applications in personalized medicine and targeted therapies.

Main Methods:

  • Utilizing various NGS platforms (Illumina, PacBio, Oxford Nanopore) for DNA and RNA sequencing.
  • Analyzing sequencing data to identify genetic variations, gene expression patterns, and epigenetic modifications.
  • Applying NGS for the discovery of disease-causing mutations and biological process regulation.

Main Results:

  • NGS has facilitated the discovery of genetic variations linked to cancer, neurological disorders, and infectious diseases.
  • Identification of key biological processes and cellular signaling pathways through NGS analysis.
  • NGS enables the development of targeted medicines and personalized treatment strategies.

Conclusions:

  • Next-generation sequencing has fundamentally transformed the field of genomics.
  • NGS is a powerful tool for understanding DNA interactions and has broad applications in medicine and environmental science.
  • The continued advancement of NGS promises further breakthroughs in biological and medical research.