Computational Inference of Gene Regulatory Network Using Genome-wide ChIP-X Data

Gene regulatory network is the architecture of transcription factors (TFs) and their gene targets, which help in controlling their expression as required by a phenotype during various environmental perturbations. Inferring the regulatory network from the high-throughput data needs an algorithmic approach involving statistical analysis. There are several interaction databases such as JASPAR and SwissRegulon that provide information for TFs-targets pair interaction, which are estimated based on experimental and prediction procedures. These repositories are majorly used for predicting the complex structure of GRNs either with or without gene expression data. Here we described and discussed the step-wise procedures to extract the interaction data for a desired set of target-TFs from the JASPAR database, and used that information to infer the network by using the igraph library. Further, we also mentioned the important parameters for analyzing the different properties of the network. The described procedure will be helpful in discerning the GRN based on the set of TF-gene pairs.