Magnetically actuated microfluidic chip combined with a G-quadruplex DNAzyme-based fluorescent/colorimetric sensor...

In this work, a portable fluorescent/colorimetric sensor based on G-quadruplex DNAzyme was constructed to achieve rapid and dual-mode detection of ochratoxin A (OTA) in wheat. OTA aptamers coupled with magnetic beads (MBs) can self-assemble with two segments of DNA and hemin to form a G-quadruplex DNAzyme structure that can catalyze the oxidation of Amplex Red (ADHP) with H₂O₂, making the solution red and producing strong fluorescence in solution. However, in the presence of OTA, the structure of the G-quadruplex DNAzyme was damaged, resulting in reduced catalytic activity. According to the principle of detection, a magnet-controlled chip integrating the reaction, washing, and detection was designed in this study. Shuttling the MB-DNAzyme probes onto a magnetically controlled chip considerably reduced the background signal and improved the detection efficiency and sensitivity. In addition, a portable fluorescence and colorimetric detection platform was built for on-site OTA detection.