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Secretion of proliferin.

S J Lee, D Nathans

    Endocrinology
    |January 1, 1987
    PubMed
    Summary

    Proliferin (PLF) is a secreted glycoprotein found in mouse cells and placenta. This protein is immunologically distinct from other known placental lactogens, offering new insights into reproductive biology.

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    Area of Science:

    • Reproductive biology
    • Molecular endocrinology
    • Cellular biology

    Background:

    • Proliferin (PLF), a member of the PRL-GH family, has mRNA detected in proliferating mouse cells and placenta.
    • Murine placental tissue suspensions produce PLF detectable by immunoprecipitation.

    Purpose of the Study:

    • To characterize the secretion and properties of proliferin (PLF) from mouse cell lines and placental tissue.
    • To determine if PLF is immunologically distinguishable from other known mouse placental lactogens.

    Main Methods:

    • Detection and characterization of PLF secretion from cultured mouse cell lines and placental tissue.
    • Immunoprecipitation assays to detect and compare PLF with other placental lactogens.
    • Analysis of the preprotein sequence to identify signal peptides.

    Main Results:

    • Proliferin (PLF) is secreted as a heterogeneous glycoprotein by growing mouse cell lines and placental tissue.
    • The initial 29 amino acids of the preprotein function as a signal peptide.
    • Placental PLF is immunologically distinct from two previously identified mouse placental lactogens.

    Conclusions:

    • Proliferin (PLF) is a secreted glycoprotein with unique immunological properties.
    • The signal peptide sequence suggests a role in protein secretion and localization.
    • These findings differentiate PLF from other placental lactogens, contributing to a better understanding of placental function.

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