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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

RNA Interference

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

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Related Experiment Video

Updated: Jul 14, 2025

Using Lipid Nanoparticles for the Delivery of Chemically Modified mRNA into Mammalian Cells

Using Lipid Nanoparticles for the Delivery of Chemically Modified mRNA into Mammalian Cells

Published on: June 10, 2022

Engineered mRNA Delivery Systems for Biomedical Applications.

Ji Wang¹, Haofang Zhu¹, Jingjing Gan¹

¹Department of Rheumatology and Immunology, Nanjing Drum Tower Hospital, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China.

Advanced Materials (Deerfield Beach, Fla.)

|October 8, 2023

Summary

Messenger RNA (mRNA) therapeutics offer a promising approach to disease treatment. Optimizing mRNA structure and delivery enhances stability and protein expression, expanding therapeutic applications.

Keywords:

biomaterials delivery systems mRNA particles therapeutics

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Last Updated: Jul 14, 2025

Using Lipid Nanoparticles for the Delivery of Chemically Modified mRNA into Mammalian Cells

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Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

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Published on: June 11, 2020

Area of Science:

Biomedical Engineering
Molecular Biology
Therapeutics Development

Background:

Messenger RNA (mRNA) therapeutics have emerged as a powerful tool for disease prevention and treatment.
Optimization of mRNA structure and delivery systems has significantly improved stability, immunogenicity, and protein expression.

Purpose of the Study:

To provide a comprehensive analysis of mRNA structure optimization strategies.
To detail advancements in mRNA delivery systems.
To summarize the latest applications of mRNA technology in biomedical engineering.

Main Methods:

Review and analysis of existing literature on mRNA structure optimization.
Detailed description of various mRNA delivery systems.
Summary of recent advancements and applications in mRNA technology.

Main Results:

Engineered mRNA modifications enhance stability and protein expression.
Diverse delivery systems are crucial for effective mRNA therapeutics.
mRNA technology shows broad applicability in infectious diseases, cancer, protein replacement, and gene editing.

Conclusions:

mRNA therapeutics hold immense potential to revolutionize disease treatment.
Continued advancements in biomedical engineering are key to overcoming challenges and realizing the full potential of mRNA therapies.