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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
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Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER
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Ultra-Fast Single-Nucleotide-Variation Detection Enabled by Argonaute-Mediated Transistor Platform.

Derong Kong1,2, Shen Zhang1,2, Mingquan Guo3

  • 1State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai, 200433, P. R. China.

Advanced Materials (Deerfield Beach, Fla.)
|October 8, 2023
PubMed
Summary
This summary is machine-generated.

Rapid single-nucleotide variation (SNV) detection is now possible using a novel graphene field-effect transistor (GFET) platform. This technology achieves high sensitivity and speed for various nucleic acid targets without amplification.

Keywords:
argonaute proteinbiosensor, field-effect transistorpoint-of-care testsingle-nucleotide variation

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Area of Science:

  • Biotechnology
  • Nanotechnology
  • Molecular Biology

Background:

  • Current single-nucleotide variation (SNV) detection methods often require lengthy procedures like nucleic acid amplification or sequencing, limiting rapid diagnostics.
  • A significant trade-off exists between the sensitivity and speed of existing SNV detection techniques, particularly for low-abundance samples.

Purpose of the Study:

  • To develop a rapid, sensitive, and specific method for "test-and-go" SNV detection.
  • To overcome the limitations of conventional SNV detection by eliminating the need for sample preparation steps like nucleic acid extraction, reverse transcription, and amplification.

Main Methods:

  • Development of a graphene field-effect transistor (GFET) platform integrated with Argonaute protein.
  • Utilizing the Argonaute protein to create a nanoscale binding channel for preorganizing DNA probes, thereby accelerating target binding.
  • Employing the GFET-Argonaute system for direct detection of SNVs in unamplified nucleic acid samples, including microRNA, circulating tumor DNA, and cDNA.

Main Results:

  • The developed GFET platform enables SNV detection with single-nucleotide resolution.
  • The system successfully identifies SNVs in the seed region of various nucleic acid targets, including tumor-associated microRNA, circulating tumor DNA, virus RNA, and reverse transcribed cDNA.
  • An integrated microchip simultaneously detected multiple SNVs within 5 minutes, achieving results comparable to sequencing.

Conclusions:

  • The Argonaute-mediated GFET platform offers a groundbreaking "test-and-go" solution for rapid and sensitive SNV detection.
  • This approach significantly reduces detection time and complexity, paving the way for fasterPoint-of-care diagnostics.
  • The technology demonstrates broad applicability for detecting SNVs in diverse biological samples without prior nucleic acid manipulation.