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Updated: Jul 14, 2025
Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
Published on: February 16, 2022
A ratiometric fluorescent indicator-displacement assay for on-site determination and intracellular imaging of
Cui-Zhen Lu1, Cai-Yun Wang1, Chao Song2
1State Key Laboratory of Digital Medical Engineering, School of Biomedical Engineering, Hainan University, Haikou 570228, China; Key Laboratory of Biomedical Engineering of Hainan Province, One Health Institute, Hainan University, Haikou 570228, China.
Abstract:
Nitroxinil (NIT) is a widely using veterinary medicine to protect cattle and sheep yet may threaten human health when ingested through food chain. Developing fluorescent analytical methods in ratiometric manners was essential for the on-site detection and in-situ monitoring of NIT but still challenging. Here, we improved the indicator-displacement assay (IDA)-based method and designed the first ratiometric fluorescent probe for NIT by using an albumin host and an Aggregation-induced emission (AIE) guest. This probe exhibited fast response (10 s), high sensitivity (limit of detection: 4.6 ppb), good selectivity (over twelve medicines) and eye-discriminable fluorescent color change (green-red) upon responding to NIT. Based on these properties, this probe enabled quantitative determination of NIT in real food samples, on-site analysis via a paper-based test strip, and fluorescence imaging of NIT in living cells.
