Oxytocin receptors in the prefrontal cortex play important roles in short-term social recognition in mice

We examined the roles of oxytocin (OT) receptors in the prefrontal cortex (PFC) in short- and long-term social recognition and anxiety-related behaviors in mice. Mice injected with high or low doses of an OT receptor antagonist (OTA) or vehicle performed the social recognition test, the open-field test, and the light-dark transition test. In the social recognition test, with three daily trials over three consecutive days, control mice showed short-term recognition of a conspecific on all three days. In contrast, a high-dose injection of OTA impaired short-term social recognition on the second and third days, and it was impaired by a low-dose injection of OTA on the third day. These results suggested that OTA injection into the PFC dose-dependently inhibited short-term social recognition within each day. All three groups did not show any long-term social recognition across three days. OTA injection did not affect anxiety related behavior in the open-field and light-dark transition tests. Our findings demonstrated that OT receptors in the PFC played important roles in short-term social recognition.