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Human basic fibroblast growth factor: nucleotide sequence and genomic organization.

J A Abraham, J L Whang, A Tumolo

    The EMBO Journal
    |October 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

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    Researchers isolated basic fibroblast growth factor (FGF) clones from human tissues. Findings suggest FGF mRNA is unstable, leading to protein storage and indicating a single gene for this important growth factor.

    Area of Science:

    • Molecular Biology
    • Biochemistry
    • Genetics

    Background:

    • Basic fibroblast growth factor (FGF) is an angiogenic endothelial cell mitogen.
    • The isolation and characterization of FGF genes are crucial for understanding cell growth and tissue repair.

    Purpose of the Study:

    • To isolate and characterize human basic FGF cDNA clones.
    • To investigate the expression levels and potential instability of basic FGF mRNA.
    • To determine the gene structure and evolutionary conservation of human basic FGF.

    Main Methods:

    • Isolation of human cDNA clones from various tissue libraries (kidney, fetal heart, fetal liver, placenta, breast carcinoma).
    • Sequence analysis of cDNA and genomic clones to deduce amino acid sequence and gene structure.

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  • Southern blot analysis of human genomic DNA to assess gene copy number.

    • Main Results:

    • Human basic FGF cDNA clones were isolated, but at low levels relative to protein quantity.
    • Evidence suggests cytoplasmic basic FGF mRNA is unstable, with protein likely stored in tissues.
    • Human basic FGF shows 99% amino acid homology to bovine basic FGF, indicating strong functional conservation.
    • Southern blot and gene mapping confirm a single basic FGF gene in humans.

    Conclusions:

    • Human basic FGF mRNA instability contributes to protein storage mechanisms.
    • The high homology between human and bovine basic FGF underscores the importance of its structure and function.
    • A single gene encodes all identified basic, heparin-binding endothelial cell mitogens, simplifying their origin.