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Effect of silencing CITED1 gene to regulate PI3K/AKT pathway on the biological function of PTC cells and its

Wanzhi Chen1, Jichun Yu2, Rong Xie3

  • 1Department of Thyroid Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 330006, China. Chenwanzhi111@163.com.

Cellular and Molecular Biology (Noisy-Le-Grand, France)
|October 9, 2023
PubMed

Insights

Silencing the CITED1 gene inhibits papillary thyroid carcinoma (PTC) cell progression by regulating the PI3K/AKT pathway. This leads to reduced cell proliferation and invasion, alongside increased apoptosis in PTC cells.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Papillary thyroid carcinoma (PTC) is a common endocrine malignancy.
  • The PI3K/AKT signaling pathway plays a crucial role in cell growth, survival, and invasion in various cancers, including PTC.
  • The role of the CITED1 gene in PTC progression and its interaction with the PI3K/AKT pathway requires further elucidation.

Purpose of the Study:

  • To investigate the effect of CITED1 gene silencing on the biological functions of human PTC cells.
  • To determine the mechanism by which CITED1 regulates the PI3K/AKT pathway in PTC.
  • To assess the impact of CITED1 silencing on cell proliferation, apoptosis, and invasion in PTC.

Main Methods:

  • Human PTC SW1736 cells were utilized and divided into control, siCITED1, LY294002 (PI3K/AKT inhibitor), and combined treatment groups.
  • CITED1 gene silencing was achieved via siCITED1 plasmid transfection.
  • Cell proliferation, apoptosis, invasion, and PI3K/AKT pathway protein/mRNA expression levels were analyzed.

Main Results:

  • Silencing CITED1 significantly decreased PTC cell proliferation and invasion while increasing apoptosis.
  • Inhibition of the PI3K/AKT pathway with LY294002 also reduced proliferation and invasion and increased apoptosis.
  • CITED1 silencing led to decreased PI3K and AKT protein expression, suggesting pathway regulation.

Conclusions:

  • CITED1 gene silencing effectively inhibits the progression of papillary thyroid carcinoma cells.
  • The inhibitory effect is mediated through the downregulation of the PI3K/AKT signaling pathway.
  • Targeting CITED1 represents a potential therapeutic strategy for PTC.

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