Related Experiment Video
Updated: Jul 14, 2025
09:44
ATAC-Seq Library Preparation of Murine Bone Marrow-Derived Neutrophils
Published on: January 3, 2025
470
Activation induces shift in nutrient utilization that differentially impacts cell functions in human neutrophils
Emily C Britt1,2, Xin Qing1,2, James A Votava1
1Morgridge Institute for Research, Madison, WI, USA.
Biorxiv : the Preprint Server for Biology
|October 9, 2023
Summary
Neutrophils primarily use glycogen for energy at rest. Upon activation, they switch to glucose, impacting functions like oxidative burst and fungal control.
Area of Science:
- Immunometabolism
- Cellular Metabolism
Background:
- Neutrophils, key innate immune cells, require significant metabolic resources for effector functions.
- Understanding neutrophil nutrient utilization is crucial for comprehending immune responses.
Approach:
- Utilized ex vivo and in vivo isotopic tracing to analyze human peripheral blood neutrophils.
- Investigated metabolic shifts in neutrophils upon activation with various stimuli.
Key Points:
- Resting neutrophils predominantly rely on glycogen for glycolysis and the pentose phosphate pathway.
- Activated neutrophils exhibit increased glucose uptake via GLUT1, making glucose the primary energy source.
- Neutrophil functions show differential nutrient dependence: oxidative burst requires glucose, while NET release is flexible.
Conclusions:
- Metabolic remodeling in neutrophils is dynamic and stimulus-dependent.
- Shifting nutrient preferences, particularly away from glycogen, enhances neutrophil migration and fungal control.
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