Qualitative and Quantitative Analysis of Azithromycin as Solid Dosage by Raman Spectroscopy

Area of Science:

• Pharmaceutical Sciences
• Analytical Chemistry
• Spectroscopy

Background:

• Active pharmaceutical ingredients (APIs) and excipients are crucial drug components requiring precise identification.
• Raman spectroscopy is a powerful, non-destructive technique for analyzing solid pharmaceutical dosage forms.

Purpose of the Study:

• To investigate the efficacy of Raman spectroscopy for the qualitative and quantitative analysis of Azithromycin in pharmaceutical formulations.
• To assess the performance of multivariate data analysis techniques, including Principal Component Analysis (PCA) and Partial Least Squares Regression (PLSR), in conjunction with Raman spectroscopy.

Main Methods:

• Raman spectroscopy was utilized to acquire spectral data from pharmaceutical formulations with varying Azithromycin concentrations and excipients.
• Principal Component Analysis (PCA) and Partial Least Squares Regression (PLSR) were applied for data analysis and model development.

Main Results:

• Raman spectroscopy successfully identified and quantified Azithromycin based on its unique spectral characteristics.
• The PLSR model demonstrated excellent performance with a goodness of fit (R²) of 0.99, RMSE calibration of 0.46, and RMSE prediction of 2.42.

Conclusions:

• Raman spectroscopy, enhanced by multivariate analysis, is a highly efficient technique for the quantitative analysis of APIs in pharmaceutical formulations.
• This approach provides a valuable tool for pharmaceutical quality control and can be adapted for various drugs and formulations.