m7GHub V2.0: an updated database for decoding the N7-methylguanosine (m7G) epitranscriptome

Area of Science:

• RNA epigenetics
• Molecular biology
• Bioinformatics

Background:

• N7-methylguanosine (m7G) RNA modification plays a crucial role in biological processes and disease pathogenesis.
• Tens of thousands of m7G sites have been identified, highlighting the need for integrated data resources.
• Previous m7GHub database focused solely on human mRNA m7G sites.

Purpose of the Study:

• To present m7GHub v.2.0, an expanded and updated resource for m7G modifications.
• To provide a comprehensive collection of m7G sites and associated variants across multiple species and RNA types.
• To facilitate sharing, annotation, and customized analysis of m7G data.

Main Methods:

• Integrated data from next-generation sequencing (NGS) and Oxford Nanopore direct RNA sequencing (ONT).
• Curated m7G sites into m7GDB (430,898 sites in 23 species).
• Collected m7G-associated variants and disease-relevant m7G-SNPs into m7GDiseaseDB (156,206 variants, 3,238 m7G-SNPs).
• Developed enhanced analysis modules: m7GFinder and m7GSNPer.

Main Results:

• m7GHub v.2.0 now includes a m7GDB with 430,898 putative m7G sites across 23 species.
• m7GDiseaseDB hosts 156,206 m7G-associated variants, including 3,238 disease-relevant m7G-SNPs.
• Enhanced analysis modules (m7GFinder, m7GSNPer) enable interactive data exploration.

Conclusions:

• m7GHub v.2.0 serves as a valuable centralized resource for m7G modification studies.
• The expanded database facilitates research into the role of m7G in various physiological and disease contexts.
• Freely accessible at www.rnamd.org/m7GHub2, promoting wider scientific engagement.