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Updated: Jul 14, 2025

Isolation and Purification of Murine Cardiac Pericytes

Isolation and Purification of Murine Cardiac Pericytes

Published on: August 16, 2019

A high-throughput single-cell RNA expression profiling method identifies human pericyte markers.

Andras Sziraki^1,2, Yu Zhong³, Allison M Neltner³

¹Laboratory of Single Cell Genomics and Population Dynamics, The Rockefeller University, New York, New York, USA.

Neuropathology and Applied Neurobiology

|October 9, 2023

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This summary is machine-generated.

Researchers identified SLC6A12 as a reliable histological marker for human brain pericytes. This new marker, detected using a commercially available antibody, improves pericyte identification in formalin-fixed paraffin-embedded (FFPE) brain tissue.

Area of Science:

Neuroscience
Cell Biology
Histology

Background:

Pericytes are crucial cells in the central nervous system vasculature.
A universally available histological marker for human brain pericytes is needed for research.
Identifying specific pericyte markers can reveal new biological functions.

Purpose of the Study:

To identify and optimize a universally available histological marker for human brain pericytes.
To discover genes specifically expressed in human pericytes.
To provide a valuable tool for researchers studying brain pericytes.

Main Methods:

Analyzed single-cell RNA expression profiles using EasySci (high-throughput, low-cost transcriptome sequencing).
Identified pericyte-specific gene markers, including SLC6A12 and SLC19A1.

Keywords:

BBB GABA SMC VCID blood-brain barrier capillaries folate neurovascular

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Validated markers using commercially available antibodies for immunohistochemistry on human FFPE brain sections and immunoblots.

Main Results:

SLC6A12 and SLC19A1 showed highly pericyte-enriched expression in EasySci data.
Antibodies against SLC6A12 and SLC19A1 recognized appropriate protein sizes in immunoblots.
Optimized immunohistochemistry with SLC6A12 antibody demonstrated superior staining of pericyte-like cells in FFPE human brain sections compared to PDGFRB.

Conclusions:

The SLC6A12 antibody is well-suited for labeling pericytes in human FFPE brain sections.
This finding is supported by integrated single-cell RNA-seq, immunoblot, and immunohistochemical data.
SLC6A12 represents a significant advancement for pericyte research in the human brain.