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Near-Infrared BODIPY Photosensitizer for Modulating Mitochondrial Fusion Proteins and Inhibiting Choroidal
Yue Li1,2, Shi-Bo Liu3, Wenjun Ni4
1Department of Ophthalmology, The Second Xiangya Hospital, Central South University, Changsha 410011, Hunan, The People's Republic of China.
ACS Applied Materials & Interfaces
|October 9, 2023
Summary
This study introduces a novel near-infrared photosensitizer (BDP2) that generates reactive oxygen species (ROS) to induce apoptosis and inhibit choroidal neovascularization, offering a new approach for photodynamic therapy (PDT).
Area of Science:
- Biochemistry
- Cell Biology
- Ophthalmology
Background:
- Photosensitizers activate cytotoxic reactive oxygen species (ROS) for photodynamic therapy (PDT), inducing apoptosis.
- Mitochondria are key players in ROS production, oxidative stress, and apoptosis.
- Near-infrared BODIPY dyes are explored as photosensitizers, but their dual role in mitochondrial function and choroidal neovascularization is underexplored.
Purpose of the Study:
- To synthesize and characterize a novel near-infrared photosensitizer (BDP2) for PDT.
- To investigate BDP2's mechanism in inducing mitochondrial dysfunction and apoptosis.
- To evaluate BDP2's efficacy in treating choroidal neovascularization.
Main Methods:
- Synthesis and characterization of a novel BODIPY-based photosensitizer (BDP2).
- Assessment of ROS generation, mitochondrial morphology changes, and apoptosis induction under near-infrared irradiation.
- Analysis of mitochondrial dynamics proteins (MFN2, OPA1) expression.
- In vivo testing of BDP2 in animal models of choroidal neovascularization.
Main Results:
- BDP2 exhibits good water solubility, long wavelength excitation, and high ROS quantum yield.
- BDP2 generates ROS, alters mitochondrial morphology, and induces apoptosis by modulating MFN2 and OPA1.
- BDP2 effectively inhibits neovascularization in animal models.
Conclusions:
- BDP2 is a potent near-infrared photosensitizer for PDT.
- BDP2 induces apoptosis via mitochondrial oxidative stress and disruption of mitochondrial dynamics.
- BDP2 demonstrates therapeutic potential for treating choroidal neovascularization.
