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Rice GA3ox1 modulates pollen starch granule accumulation and pollen wall development.

Kun-Ting Hsieh1,2, Chi-Chih Wu2, Shih-Jie Lee1

  • 1Institute of Molecular Biology, National Chung Hsing University, Taichung, Taiwan.

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|October 9, 2023
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Summary

The rice GA3ox1 gene is essential for pollen development, impacting starch accumulation and pollen wall formation. Mutations in OsGA3ox1 lead to reduced seed set and pollen viability in rice plants.

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Area of Science:

  • Plant Molecular Biology
  • Reproductive Biology
  • Genetics

Background:

  • The gibberellin (GA) biosynthetic gene OsGA3ox1 in rice is suggested to regulate gametophytic pollen development.
  • However, detailed cellular characterization of its mutant pollen has been limited.

Purpose of the Study:

  • To functionally characterize the OsGA3ox1 gene's role in rice pollen development using CRISPR/Cas9 gene editing.
  • To investigate the cellular and molecular mechanisms underlying pollen defects in osga3ox1 mutants.

Main Methods:

  • CRISPR/Cas9 gene editing was employed to generate biallelic variants of the OsGA3ox1 gene.
  • Homozygous mutants were analyzed for effects on vegetative and floral organ development, seed-setting rate, and pollen viability.
  • Anatomical and molecular characterizations were performed on mutated pollen.

Main Results:

  • Homozygous osga3ox1 mutants exhibited reduced seed-setting rates and pollen viability without affecting most vegetative and floral organs.
  • Mutant pollen showed defects in starch granule accumulation and pollen wall development.
  • Abnormal pollen development was linked to the regulation of transcription factors OsGAMYB, OsTDR, and OsbHLH142.

Conclusions:

  • The rice GA3ox1 gene plays a critical role in modulating pollen starch granule accumulation and pollen wall development during the gametophytic phase.
  • OsGA3ox1 is crucial for male gametophyte development and fertility in rice.