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Quercetin-targeted AKT1 regulates the Raf/MEK/ERK signaling pathway to protect against doxorubicin-induced

Wang Yufang1, Liu Mingfang2, Huang Nan1

  • 1Department of Medical Laboratory Diagnosis Lecturer, Quanzhou Medical College, Quanzhou, Fujian Province, China.

Tissue & Cell
|October 9, 2023
PubMed
Abstract

Insights

Quercetin protects against doxorubicin-induced kidney injury in mice by reducing inflammation and improving renal function. This natural compound may offer a therapeutic strategy for nephrotoxicity.

Area of Science:

  • Pharmacology
  • Nephrology
  • Biochemistry

Background:

  • Doxorubicin (anticancer drug) causes kidney damage (nephrotoxicity), necessitating research into protective agents.
  • Mouse models of doxorubicin-induced nephropathy are crucial for preclinical studies.
  • Quercetin is investigated for its potential renoprotective effects.

Purpose of the Study:

  • To evaluate the protective effect of quercetin against doxorubicin-induced kidney injury in a mouse model.
  • To elucidate the underlying mechanisms of quercetin's action on renal function and cellular pathways.

Main Methods:

  • Doxorubicin-induced nephropathy model in C57BL/6 mice.
  • Assessment of kidney function markers (serum and urine).
  • Histopathology (H&E), electron microscopy, immunohistochemistry, RT-qPCR, ELISA, Western blot, and molecular docking.

Main Results:

  • Quercetin treatment improved kidney function and reduced tissue damage and fibrosis.
  • Mitochondrial structure and apoptotic protein levels (Bax, Bcl-2) were normalized by quercetin.
  • Quercetin downregulated Ang II, inflammatory factors, and modulated the AKT1/Raf/MEK/ERK pathway.

Conclusions:

  • Quercetin demonstrates significant renoprotective effects against doxorubicin-induced nephropathy.
  • The protective mechanism involves regulating inflammatory factors and the AKT1/Raf/MEK/ERK signaling pathway.
  • Quercetin shows promise as a therapeutic agent for mitigating doxorubicin-induced kidney injury.
Keywords:
AKT1Kidney injuryMolecular dockingQuercetinRaf/MEK/ERK signaling pathway

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