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Related Experiment Videos

Interactive responses to diethylstilboestrol in C3H mice.

D L Greenman, R R Delongchamp

    Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
    |September 1, 1986
    PubMed
    Summary

    Diethylstilbestrol (DES) exposure in mice revealed varying sensitivities. Some mice showed increased uterine and vaginal effects with DES, while mammary tumor development decreased as uterine hyperplasia severity increased.

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    Area of Science:

    • Endocrinology
    • Toxicology
    • Oncology

    Background:

    • Diethylstilbestrol (DES) is a synthetic estrogen with known endocrine-disrupting properties.
    • DES exposure can affect various estrogen-sensitive organs, including the uterus, vagina, bone, and mammary glands.
    • Individual variability in response to endocrine disruptors is a critical area of research.

    Purpose of the Study:

    • To investigate the differential effects of dietary DES on estrogen-sensitive organs in female C3HeB/FeJ mice.
    • To determine if specific uterine responses correlate with varying sensitivities in other estrogen-sensitive tissues.
    • To explore the relationship between uterine glandular hyperplasia severity and the incidence of mammary tumors.

    Main Methods:

    • Female C3HeB/FeJ mice were fed a diet containing 100 ppb DES from 6 weeks of age.

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  • Histological examination of target organs (uterus, vagina, bone, mammary gland) was performed on mice that died, became moribund, or were killed at scheduled intervals or due to mammary tumors.
  • Matched-pair analysis was used to compare outcomes between mice with different grades of uterine glandular hyperplasia and control mice without hyperplasia.

    • Main Results:

    • Mice with uterine glandular hyperplasia exhibited increased prevalence/severity of uterine fibrosis, vaginal mucoid stroma, and vaginal keratinization compared to controls.
    • These uterine and vaginal effects intensified with increasing hyperplasia severity.
    • Osseous trabecular proliferation and marrow fibrosis prevalence were consistent across all hyperplasia grades.
    • Mammary adenocarcinoma prevalence was inversely related to uterine glandular hyperplasia severity.

    Conclusions:

    • A subpopulation of mice exhibits heightened sensitivity to DES for uterine and vaginal endpoints.
    • This sensitive subpopulation demonstrates reduced susceptibility to DES-induced mammary tumor formation.
    • These findings highlight the complex and organ-specific responses to endocrine disruptors like DES.