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Related Concept Videos

Overview Of Cell Separation And Isolation01:20

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Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
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Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
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Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
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Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
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Using dielectrophoretic spectra to identify and separate viable yeast cells.

Sakshin Bunthawin1, Paphawarin Srichan1, Kata Jaruwongrungsee2

  • 1Biotechnology of Electromechanics Research Unit, Faculty of Technology and Environment, Prince of Songkla University, Kathu, Phuket, 83120, Thailand.

Applied Microbiology and Biotechnology
|October 10, 2023
PubMed
Summary

Dielectrophoresis effectively separates viable and non-viable yeast cells by analyzing their distinct dielectric properties. This method offers a scalable solution for assessing cell viability in the fermentation industry.

Keywords:
Cell dielectric propertiesCytoplasmic conductivityDielectrophoresisSignal generatorViabilityViable cell separationYeast cell

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Area of Science:

  • Biophysics
  • Biotechnology
  • Food Science

Background:

  • Assessing yeast cell viability is crucial for the fermentation and brewery industries.
  • Traditional methods can be time-consuming and may not offer real-time analysis.
  • Dielectrophoresis (DEP) presents a potential non-invasive method for cell analysis.

Purpose of the Study:

  • To investigate the use of dielectrophoresis for distinguishing between viable and non-viable yeast cells.
  • To analyze the dielectric properties of yeast cells to determine viability.
  • To evaluate the potential of DEP for cell separation in industrial fermentation.

Main Methods:

  • Yeast cells (Saccharomyces cerevisiae) were subjected to non-uniform sinusoidal electric fields using micro-parallel cylindrical electrodes.
  • Cell viability was assessed by analyzing dielectrophoretic spectra and lower critical frequencies (LCF) at varying medium conductivities.
  • Real-time image processing and a sequential signal generator (SSG) were used to monitor cell movement and collection yield.

Main Results:

  • Living and dead yeast cells exhibited distinct dielectrophoretic spectra and dielectric properties.
  • The cytoplasmic conductivity (σc) of living yeast cells was approximately 0.2 S m⁻¹, significantly higher than that of dead cells (≤ 0.05 S m⁻¹).
  • Positive dielectrophoresis (pDEP) was observed, with LCF values shifting based on medium conductivity.

Conclusions:

  • Dielectrophoresis is a viable method for differentiating and separating viable from non-viable yeast cells.
  • Cellular dielectric properties, particularly cytoplasmic conductivity, serve as reliable indicators of yeast cell viability and metabolic health.
  • This technique offers a scalable and real-time approach for quality control in the fermentation industry.