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Related Experiment Videos

[Lymphocyte transformation test in the calf].

W Methling, G Mehlhorn

    Archiv Fur Experimentelle Veterinarmedizin
    |January 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

    The lymphocyte transformation test accurately assesses calf immune responses to environmental factors. This method is valuable for understanding how farming conditions impact animal health and performance.

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    Area of Science:

    • Veterinary Immunology
    • Animal Health
    • Environmental Science

    Background:

    • Environmental factors significantly impact agricultural animal health and performance.
    • Assessing immunological reactivity is crucial for understanding animal well-being under various conditions.
    • Lymphocyte transformation is a key indicator of immune system status.

    Purpose of the Study:

    • To report methodical experience in isolating and culturing calf lymphocytes.
    • To evaluate the suitability of the lymphocyte transformation test for assessing environmental impacts.
    • To determine the precision of morphological evaluation in lymphocyte transformation.

    Main Methods:

    • Lymphocyte isolation from defibrinated calf blood using Visotrast-370 centrifugation.

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  • Lymphocyte culturing over three days in a medium of autologous serum and Eagle medium with antibiotics.
  • Addition of phytohaemagglutinin (PHA) to stimulate lymphocyte transformation.
  • Morphological evaluation of lymphocyte transformation for precision.

    • Main Results:

    • Visotrast-370 centrifugation is recommended for optimal lymphocyte isolation.
    • Specific culturing conditions (1x10^6 cells/mL, 20% autologous serum, 80% Eagle medium, PHA) ensure high precision.
    • Morphological evaluation provides accurate assessment of lymphocyte transformation.

    Conclusions:

    • The lymphocyte transformation test is methodically accurate for detecting environmental effects on calf immunological reactivity.
    • This test can identify specific environmental impacts on the immune system.
    • The test likely reflects the immunological response to antigen exposure, offering insights into animal health.