Rapid and easily identifiable blood typing on microfluidic cotton thread-based analytical devices

In this study, we present a novel swing-elution-based method to achieve rapid, cost-effective, and easily identifiable blood typing assays. Specifically, the method aims to swing the microfluidic cotton thread-based analytical devices (μCTADs) in PBS solution to effectively elute free red blood cells (RBCs) and allow large agglutinated RBCs to remain to precisely determine the blood type. In order to ensure an easily identifiable blood typing assay, fast swing mode needs to be used, and the elution time is evaluated to be >50 seconds. The created μCTADs have been used to successfully classify ABO and RhD blood types in 56 blood samples. Finally, in order to enhance the convenience and portability of blood typing, a blood-typing chip that utilizes a PBS liquid bridge to effectively elute the free RBCs is designed and fabricated based on the above swing-elution principle. Compared with the traditional wicking-elution methods that rely on the wicking effect to weakly elute the RBCs, our method possesses a stronger elution effect to remove the free RBCs inside the inter-fiber gaps or adhered to the fiber surface, resulting in effectively enhancing the identifiability of the elution results and minimizing user interpretation error. Given the simplicity of the blood typing method, we believe that our blood typing method has great potential to be widely applied in resource-limited and developing regions.