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Optimized HPLC extraction method of quercetin and berberine based on response surface analysis
LanQing Li1, Jia Cheng1, Fan Lu1
1Department of Resources and Environment, Anhui Science and Technology University Fengyang 233100 China xiey@ahstu.edu.cn.
RSC Advances
|October 11, 2023
Summary
This study introduces a rapid HPLC-PDA method for simultaneously determining quercetin and berberine in soil. Optimized ultrasonic extraction achieved high efficiency, offering a practical tool for soil allelochemical analysis.
Area of Science:
- Environmental Chemistry
- Analytical Chemistry
Background:
- Quercetin and berberine are important soil allelochemicals.
- Accurate determination and extraction methods are crucial for soil analysis.
Purpose of the Study:
- To develop a simultaneous determination and extraction method for quercetin and berberine in soil.
- To optimize extraction conditions using the response surface method.
Main Methods:
- High-Performance Liquid Chromatography with Photodiode Array (HPLC-PDA) detection.
- Multi-wavelength detection at 210 nm and 347 nm.
- Response surface methodology for optimizing ultrasonic extraction.
Main Results:
- Complete separation of quercetin and berberine within 20 minutes.
- Low detection limits (quercetin: 0.078 mg L⁻¹, berberine: 0.019 mg L⁻¹).
- High recovery rates (97.2%–107.4%) and optimized extraction conditions (44°C, 10 min, 80% ethanol, 1:17 solid-liquid ratio).
Conclusions:
- The developed method is simple, fast, cost-effective, and safe for soil sample analysis.
- The method is suitable for practical applications and provides a valuable reference for future research on soil allelochemicals.
