Related Experiment Video
Updated: Jul 13, 2025

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues
Published on: February 19, 2019
A Pseudomonas aeruginosa-Suitable Fluorescent Reporter System for Analyzing Small RNA-Mediated Regulation of Target
Silvia Santoro1, Giovanni Bertoni2, Silvia Ferrara3
1Department of Biosciences, Università degli Studi di Milano, Milan, Italy.
Abstract:
Bacterial small RNAs have emerged as modulators that play key roles in regulatory networks related to viability, environmental adaptation, and pathogenesis. Small RNAs can modulate gene expression by base-pairing to target mRNAs, influencing their translation and/or stability. A superfolder GFP reporter system, previously developed for Escherichia coli and Salmonella enterica, was adapted to Pseudomonas aeruginosa and used to validate novel mRNA targets in studies of small RNA-mediated regulatory mechanisms.
More Related Videos
Related Concept Videos
Reporter Genes
Translational Regulation
Gene Regulation in Microbial Communities: Quorum Sensing

