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CRISPR/Cas and Argonaute-Based Biosensors for Pathogen Detection

Zhiruo Yang1, Siying Mao1, Lu Wang1

  • 1Hubei Province Key Laboratory of Occupational Hazard identification and Control, School of Medicine, School of Public Health, Wuhan University of Science and Technology, Wuhan 430065, PR China.

ACS Sensors
|October 11, 2023
PubMed

Insights

Rapidly identifying pathogens is crucial for public health security. This review compares CRISPR/Cas systems and Argonaute (Ago) for developing advanced pathogen detection biosensors.

Area of Science:

  • Microbiology
  • Biotechnology
  • Biosensor Technology

Background:

  • Pathogen detection is critical for preventing public health crises.
  • Existing methods require highly sensitive and specific pathogen identification approaches.
  • Microbial Defense Systems (MDS) like CRISPR/Cas and Argonaute (Ago) offer novel solutions.

Purpose of the Study:

  • To compare CRISPR/Cas systems and Argonaute (Ago) for pathogen detection.
  • To review the similarities and differences between these two nucleases.
  • To discuss future prospects for CRISPR/Cas and Ago-based biosensors, particularly electrochemical biosensors.

Main Methods:

  • Comparative analysis of CRISPR/Cas and Argonaute (Ago) mechanisms.
  • Literature review on microbial defense systems and biosensor applications.
  • Exploration of electrochemical biosensor development for pathogen detection.

Main Results:

  • CRISPR/Cas and Ago are programmable nucleases from Microbial Defense Systems.
  • Both systems enable guided, targeted activation for pathogen identification.
  • Electrochemical biosensors show promise for sensitive and specific pathogen detection.

Conclusions:

  • CRISPR/Cas and Ago represent a new generation of pathogen detection tools.
  • Further development of CRISPR/Cas and Ago-based electrochemical biosensors is needed.
  • This review provides guidance for researchers in the field of pathogen detection biosensors.
Keywords:
ArgonauteCRISPR/Casbiosensorelectrochemical detectionmicrobial defense systemmolecular diagnosticspathogensensing

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