Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Review of Activator Strand Engineering Strategies for Smart CRISPR/Cas12a Diagnostics.

ACS sensors·2026
Same author

Molecular engineering of CRISPR/Cas12a: from activity enhancement to exponential signal amplification.

Chemical science·2026
Same author

An allosteric key strand controlled adaptable CRISPR/Cas12a biosensing platform for point-of-care testing of multiple types of targets.

Lab on a chip·2026
Same author

Dual Fe-Active-Site Engineering in MCOF Nanozymes for Synergistic and Broad-Spectrum Antibacterial Therapy.

Small (Weinheim an der Bergstrasse, Germany)·2025
Same author

Super-Resolution Axial Imaging for Quantifying Piconewton Traction Forces in Live Cells.

Angewandte Chemie (International ed. in English)·2025
Same author

5'-End Engineering of CRISPR/Cas12a Activators: A Versatile Platform for Multiple Biomarker Analysis and Clinical Cancer Tissue Identification.

Analytical chemistry·2025

Related Experiment Video

Updated: Jun 17, 2026

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
09:03

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Published on: December 23, 2022

2.7K

Low-Background CRISPR/Cas12a Sensors for Versatile Live-Cell Biosensing.

Qing-Nan Li1, Dong-Xia Wang1, Gui-Mei Han1

  • 1State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Research Centre for Analytical Sciences, College of Chemistry, Nankai University, Tianjin 300071, P. R. China.

Analytical Chemistry
|October 11, 2023
PubMed
Summary

Researchers developed a novel CRISPR/Cas12a biosensor using a "RESET" activator strand to overcome high background issues. This innovation enables sensitive detection in live cells and point-of-care applications.

More Related Videos

Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging
04:33

Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging

Published on: December 8, 2023

913
Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
10:16

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

1.2K

Related Experiment Videos

Last Updated: Jun 17, 2026

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
09:03

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Published on: December 23, 2022

2.7K
Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging
04:33

Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging

Published on: December 8, 2023

913
Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
10:16

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

1.2K

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Biosensing

Background:

  • CRISPR/Cas12a's trans-cleavage activity is vital for biosensing.
  • Existing "on-off-on" CRISPR/Cas12a biosensors face high background noise, limiting intracellular use.

Purpose of the Study:

  • To overcome high background in CRISPR/Cas12a biosensors.
  • To enable sensitive detection and intracellular applications.
  • To enhance biosensor flexibility for diverse targets.

Main Methods:

  • Designed a CRISPR/Cas12a activator strand (AS) utilizing the "RESET" effect for controllable activation.
  • Developed strategies including strand displacement, cleavage, and aptamer-substrate interactions to reactivate inhibited Cas12a activity.
  • Integrated biosensors with lateral flow assay (LFA) for point-of-care testing.

Main Results:

  • Achieved low background noise by inhibiting AS activation.
  • Demonstrated sensitive detection of nucleic acid (miR-21, 0.96 pM), ATP (8.6 μM), and hOGG1 (8.3 × 10-5 U/mL).
  • Successfully performed intracellular imaging of biomolecules and integrated with LFA.

Conclusions:

  • The "RESET" effect significantly improves CRISPR/Cas12a biosensor performance and flexibility.
  • Developed biosensors are suitable for live-cell analysis and point-of-care diagnostics.
  • This approach broadens the applicability of CRISPR/Cas12a in various sensing scenarios.