Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 13, 2025

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects
08:48

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects

Published on: April 21, 2022

2.5K

Improved age estimation from semen using sperm-specific age-related CpG markers.

Chao Xiao1, Ya Li2, Maomin Chen2

  • 1Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, PR China; Hubei Key Laboratory of the Forensic Science, Hubei University of Police, Wuhan, Hubei 430035, PR China.

Forensic Science International. Genetics
|October 11, 2023
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Applicability of imprinted DIP-SNP markers in DNA mixture analysis: a pilot study.

International journal of legal medicine·2026
Same author

DNA methylation-based multi-tissue age prediction model for blood, saliva, and buccal swab samples.

International journal of legal medicine·2026
Same author

Study on the floor heave mechanism and control technology for the gob-side roadway in the stopping mining area of thick coal seam.

Scientific reports·2026
Same author

A galactic cosmic ray cavity in Earth-Moon space.

Science advances·2026
Same author

Sentinel lymph node biopsy versus lymphadenectomy in early-stage cervical cancer: a meta-analysis of oncologic outcomes and surgical morbidity.

Frontiers in oncology·2026
Same author

Efficacy and safety of immune checkpoint inhibitors combined with chemoradiotherapy in locally advanced cervical cancer: a systematic review and meta-analysis.

Frontiers in pharmacology·2026

This study developed sperm-specific age-related CpG markers for accurate age estimation from sperm DNA, improving forensic investigations. The new method offers a more precise tool for narrowing suspect pools in sexual assault cases.

Area of Science:

  • Forensic Science
  • Genetics
  • Molecular Biology

Background:

  • Accurate age estimation from semen is crucial for sexual assault investigations.
  • Previous semen age estimation methods using age-related CpG (AR-CpG) markers were less accurate than blood-based methods.
  • Interference from non-sperm cells in semen likely caused lower accuracy in prior studies.

Purpose of the Study:

  • To develop age calculators using sperm-specific AR-CpG markers.
  • To improve age estimation accuracy from sperm DNA.
  • To provide a more reliable forensic tool for age determination.

Main Methods:

  • Analyzed public MethylationEPIC microarray data from 90 sperm samples (ages 22-51).
  • Identified 31 sperm-specific AR-CpG markers and integrated top markers into two SNaPshot panels.
Keywords:
Age estimationCpG markerDNA methylationForensic scienceSemen

More Related Videos

Methods to Study Changes in Inherent Protein Aggregation with Age in Caenorhabditis elegans
11:57

Methods to Study Changes in Inherent Protein Aggregation with Age in Caenorhabditis elegans

Published on: November 26, 2017

8.7K
Accurate and Phenol Free DNA Sexing of Day 30 Porcine Embryos by PCR
10:16

Accurate and Phenol Free DNA Sexing of Day 30 Porcine Embryos by PCR

Published on: February 14, 2016

10.1K

Related Experiment Videos

Last Updated: Jul 13, 2025

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects
08:48

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects

Published on: April 21, 2022

2.5K
Methods to Study Changes in Inherent Protein Aggregation with Age in Caenorhabditis elegans
11:57

Methods to Study Changes in Inherent Protein Aggregation with Age in Caenorhabditis elegans

Published on: November 26, 2017

8.7K
Accurate and Phenol Free DNA Sexing of Day 30 Porcine Embryos by PCR
10:16

Accurate and Phenol Free DNA Sexing of Day 30 Porcine Embryos by PCR

Published on: February 14, 2016

10.1K
  • Validated 21 AR-CpG markers in an independent cohort of 253 sperm DNA samples (ages 22-67).

    • Main Results:

    • Developed optimal age estimation models for sperm DNA with mean absolute errors (MAEs) as low as 2.526 years.
    • Simplified models with fewer markers still outperformed previous methods (e.g., MAE of 2.918 years for sperm).
    • Markers demonstrated 100% accuracy in distinguishing sperm/semen from blood.

    Conclusions:

    • Sperm-specific AR-CpG markers enable precise age estimation from sperm DNA.
    • The developed markers and models offer an improved toolset for forensic science.
    • This research enhances the potential for accurate age determination in legal investigations.