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Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink
Published on: April 21, 2016
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Engineering dense tumor constructs via cellular contraction of extracellular matrix hydrogels
Jae A McKee1,2, Elisabet A Olsen1,2, Gideon Wills Kpeli1
1Department of Biomedical Engineering, Tulane University, New Orleans, Louisiana, USA.
Biotechnology and Bioengineering
|October 12, 2023
Summary
Researchers engineered dense tumor models using human fibroblasts and carcinoma cells. This novel approach mimics solid tumors
Area of Science:
- Biomedical Engineering
- Cancer Research
- Tissue Engineering
Background:
- Solid tumors exhibit physical characteristics like dense microarchitectures and stiffness, impacting cancer progression.
- Existing engineered models often lack the complex internal microarchitecture of tumors, such as compacted extracellular matrix (ECM) fibers.
Purpose of the Study:
- To engineer a tissue construct that accurately mimics the physical characteristics of dense solid tumors in vivo.
- To develop a tractable model for studying cancer pathophysiology and therapeutic delivery challenges.
Main Methods:
- Human fibroblasts were used to contract collagen type I hydrogels co-seeded with carcinoma cell spheroids.
- Morphometry, mechanical testing, and biochemical analysis (proliferation, viability) were employed.
Main Results:
- The engineered constructs successfully mimicked the dense microarchitecture and physical properties of solid tumors.
- The model preserved cell viability and was suitable for extended culture.
- The method is adaptable for prevascularized constructs using endothelial cells and fibrin.
Conclusions:
- This method provides a clinically relevant platform for studying solid tumor pathophysiology.
- Engineered dense carcinoma constructs offer a valuable tool for investigating challenges in drug and immunotherapy delivery.
