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Light Sheet-Based Laser Patterning Bioprinting Produces Long-Term Viable Full-Thickness Skin Constructs
Levin Hafa1, Louise Breideband1, Lucas Ramirez Posada1
1Institute of Cell Biology and Neurosciences (IZN), Buchman Institute for Molecular Life Sciences (BMLS), Goethe-Universität Frankfurt am Main, Max-von-Laue-Straße 15, 60438, Frankfurt am Main, Germany.
Advanced Materials (Deerfield Beach, Fla.)
|October 12, 2023
Summary
This study introduces a novel light sheet bioprinting system for rapid, high-resolution tissue engineering. The technology enables real-time imaging and the creation of viable skin constructs, advancing regenerative medicine.
Area of Science:
- Biomedical Engineering
- Tissue Engineering
- Regenerative Medicine
Background:
- Tissue engineering offers alternatives to animal models for biomedical research and healthcare.
- 3D bioprinting provides design flexibility and reproducibility in tissue fabrication.
- Existing bioprinting methods face limitations in speed and real-time imaging capabilities.
Purpose of the Study:
- To develop an integrated fluorescent light sheet bioprinting and imaging system.
- To achieve high printing speed and resolution with real-time monitoring.
- To demonstrate the system's capability in creating functional tissue constructs.
Main Methods:
- Utilized direct laser patterning and a static light sheet for precise photocrosslinking.
- Integrated fluorescent recovery after photobleaching (FRAP) and brightfield imaging for hydrogel monitoring.
- Employed in situ light sheet imaging for observing encapsulated cells.
Main Results:
- Achieved high printing speed (0.66 mm³/s) and resolution (9 µm).
- Demonstrated high viability (83% ± 4.34%) and functionality of encapsulated human fibroblasts.
- Successfully fabricated full-thickness skin constructs viable for 41 days.
Conclusions:
- The integrated light sheet bioprinting system offers high speed, resolution, and real-time characterization capabilities.
- This technology advances tissue engineering by enabling the creation of complex and viable tissue constructs.
- Future enhancements promise further improvements in resolution and speed for light-based bioprinting applications.
